FIGURE

Fig. 2

ID

ZDB-FIG-160209-1

Publication

Voelz et al., 2015 - A zebrafish larval model reveals early tissue-specific innate immune responses to Mucor circinelloides

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Phagocytes are recruited to the site of infection after spore injection into the hindbrain and interact with spores in vivo. Macrophage (A) and neutrophil recruitment (B) to the site of spore injection in the hindbrain ventricle of Tg(mpeg1:G/U:NfsB-mCherry) and Tg(mpx:GFP) zebrafish larvae, respectively, was observed at 4 and 24h.p.i. Pooled data presented were obtained from three independent experimental repeats with 10 larvae each. (C) Spores can be observed inside macrophages (indicated by asterisks) and neutrophils (indicated by arrowheads) after injection of viable and non-viable spores in the hindbrain ventricle of Tg(mpeg1:G/U:NfsB-mCherry/mpx:GFP) larvae (representative images at 10h 45min and 1h 5min, respectively; z-stack: 15 sections every 7.3µm; scale bar: 20µm). (D) Several subsequent events of spore phagocytosis were also observed in an undefined phagocyte in vivo (arrow indicates next spore to be phagocytosed; time is expressed as h:min:s; scale bar: 4µm; see also Movie 1).

Expression Data

Genes:	GFP mCherry
Fish:	c264Tg; gl25Tg; i114Tg
Condition:	fungal treatment by injection: Mucor circinelloides
Anatomical Terms:	macrophage neutrophil
Stage Range:	Prim-25 to High-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	i114Tg c264Tg; gl25Tg
Condition:	fungal treatment by injection: Mucor circinelloides
Observed In:	hindbrain macrophage hindbrain neutrophil
Stage Range:	Prim-25 to Pec-fin

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.