ZFIN ID: ZDB-FIG-151203-12 |
Fig. 3 Phagocytes accumulate at the site of live spore injection in the hindbrain infection model. (A) Macrophages and neutrophils accumulate around viable spores (z-stack: 15 sections every 7.3µm; scale bar: 40µm; see also Movie 2). (B) Significantly less macrophage and neutrophil clustering was observed in larvae injected with UV-killed spores (z-stack: 15 sections every 7.3µm; scale bar: 40µm; see also Movie 3). (C) Formation of clusters around viable spores was correlated with low levels of spore dissemination, whereas non-viable spores did disseminate efficiently throughout the fish (z-stacks: 30 sections every 10.35 and 9.25µm, respectively, scale bars: 50µm; zoomed in section: z-stack 31 sections every 1µm). Time is expressed as h:min:s starting at 1h after injection. |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
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GFP | c264Tg; gl25Tg; i114Tg | fungal treatment by injection: Mucor circinelloides | Prim-25 to High-pec | neutrophil | IFL | |
mCherry | c264Tg; gl25Tg | fungal treatment by injection: Mucor circinelloides | Prim-25 to High-pec | macrophage | IFL | |
c264Tg; gl25Tg; i114Tg | fungal treatment by injection: Mucor circinelloides | Prim-25 to High-pec | macrophage | IFL |
Acknowledgments: | This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech. |
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