FIGURE

Fig. 3

ID

ZDB-FIG-151203-12

Publication

Voelz et al., 2015 - A zebrafish larval model reveals early tissue-specific innate immune responses to Mucor circinelloides

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Fig. 3

Phagocytes accumulate at the site of live spore injection in the hindbrain infection model. (A) Macrophages and neutrophils accumulate around viable spores (z-stack: 15 sections every 7.3�m; scale bar: 40�m; see also Movie 2). (B) Significantly less macrophage and neutrophil clustering was observed in larvae injected with UV-killed spores (z-stack: 15 sections every 7.3�m; scale bar: 40�m; see also Movie 3). (C) Formation of clusters around viable spores was correlated with low levels of spore dissemination, whereas non-viable spores did disseminate efficiently throughout the fish (z-stacks: 30 sections every 10.35 and 9.25�m, respectively, scale bars: 50�m; zoomed in section: z-stack 31 sections every 1�m). Time is expressed as h:min:s starting at 1h after injection.

Expression Data

Genes:	GFP mCherry
Fish:	c264Tg; gl25Tg c264Tg; gl25Tg; i114Tg
Condition:	fungal treatment by injection: Mucor circinelloides
Anatomical Terms:	macrophage neutrophil
Stage Range:	Prim-25 to High-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	c264Tg; gl25Tg c264Tg; gl25Tg; i114Tg
Condition:	fungal treatment by injection: Mucor circinelloides
Observed In:	hindbrain hindbrain macrophage hindbrain neutrophil macrophage neutrophil
Stage Range:	Prim-25 to High-pec

Phenotype Detail

Acknowledgments

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