ZFIN ID: ZDB-FIG-151203-12
Voelz et al., 2015 - A zebrafish larval model reveals early tissue-specific innate immune responses to Mucor circinelloides. Disease models & mechanisms   8(11):1375-88 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-25 to High-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-25 to High-pec

Fig. 3

Phagocytes accumulate at the site of live spore injection in the hindbrain infection model. (A) Macrophages and neutrophils accumulate around viable spores (z-stack: 15 sections every 7.3µm; scale bar: 40µm; see also Movie 2). (B) Significantly less macrophage and neutrophil clustering was observed in larvae injected with UV-killed spores (z-stack: 15 sections every 7.3µm; scale bar: 40µm; see also Movie 3). (C) Formation of clusters around viable spores was correlated with low levels of spore dissemination, whereas non-viable spores did disseminate efficiently throughout the fish (z-stacks: 30 sections every 10.35 and 9.25µm, respectively, scale bars: 50µm; zoomed in section: z-stack 31 sections every 1µm). Time is expressed as h:min:s starting at 1h after injection.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP c264Tg; gl25Tg; i114Tg fungal treatment by injection: Mucor circinelloides Prim-25 to High-pec neutrophil IFL
mCherry c264Tg; gl25Tg fungal treatment by injection: Mucor circinelloides Prim-25 to High-pec macrophage IFL
c264Tg; gl25Tg; i114Tg fungal treatment by injection: Mucor circinelloides Prim-25 to High-pec macrophage IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
c264Tg; gl25Tg fungal treatment by injection: Mucor circinelloides Prim-25 - High-pec macrophage accumulation hindbrain, abnormal
c264Tg; gl25Tg; i114Tg fungal treatment by injection: Mucor circinelloides Prim-25 - High-pec hindbrain macrophage increased amount, abnormal
Prim-25 - High-pec hindbrain neutrophil increased amount, abnormal
Prim-25 - High-pec macrophage accumulation hindbrain, abnormal
Prim-25 - High-pec neutrophil accumulation hindbrain, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.