Genetic knockdown of zebrafish nos2b and sGC (gucy) but not nos1 and nos2a impair the inflammatory response.
Assessment of the role of the 3 zebrafish Nos isoforms and sGC on peripheral sensory nervous system (a) and epithelial (b) inflammation upon genetic knockdown. (a) nos2b and gucy knockdown significantly reduce initial inflammation at injured neuromasts as compared to wildtype (WT) larvae. (b) Knockdown of nos2b and gucy significantly reduce the number of neutrophils recruited to ventral fin wounds. Bar charts represent means of the initial inflammatory index (%) ± SEM 90 minutes after CuSO4 treatment (a) and 1 h after mechanical injury of the ventral fin (b). Three individual experiments were performed with 15 replicate morphant larvae for each condition, respectively. Statistics were evaluated with unpaired one-sided t-tests. ns–not significant: P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001.