ZFIN ID: ZDB-FIG-160202-16
Wittmann et al., 2015 - A Zebrafish Drug-Repurposing Screen Reveals sGC-Dependent and sGC-Independent Pro-Inflammatory Activities of Nitric Oxide. PLoS One   10:e0137286 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 4

Genetic knockdown of zebrafish nos2b and sGC (gucy) but not nos1 and nos2a impair the inflammatory response.

Assessment of the role of the 3 zebrafish Nos isoforms and sGC on peripheral sensory nervous system (a) and epithelial (b) inflammation upon genetic knockdown. (a) nos2b and gucy knockdown significantly reduce initial inflammation at injured neuromasts as compared to wildtype (WT) larvae. (b) Knockdown of nos2b and gucy significantly reduce the number of neutrophils recruited to ventral fin wounds. Bar charts represent means of the initial inflammatory index (%) ± SEM 90 minutes after CuSO4 treatment (a) and 1 h after mechanical injury of the ventral fin (b). Three individual experiments were performed with 15 replicate morphant larvae for each condition, respectively. Statistics were evaluated with unpaired one-sided t-tests. ns–not significant: P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: copper(II) sulfate Protruding-mouth inflammatory response to wounding increased process quality, abnormal
WT + MO1-gucy1a1 chemical treatment: copper(II) sulfate Protruding-mouth inflammatory response to wounding increased process quality, ameliorated
WT + MO1-nos2b chemical treatment: copper(II) sulfate Protruding-mouth inflammatory response to wounding increased process quality, ameliorated
nz50Tg laceration: ventral fin fold Protruding-mouth inflammatory response to wounding increased process quality, abnormal
nz50Tg + MO1-gucy1a1 laceration: ventral fin fold Protruding-mouth inflammatory response to wounding increased process quality, ameliorated
nz50Tg + MO1-nos2b laceration: ventral fin fold Protruding-mouth inflammatory response to wounding increased process quality, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One