Fig. 3

Nitric oxide is a pro-inflammatory signal upon peripheral sensory nervous system and epithelial tissue injuries.

Inhibition of NOS and sGC significantly impairs leukocyte-to-wound attraction in both inflammation scenarios. (a) Schematic of the NO signaling pathway. Relevant chemical inhibitors acting on individual pathway components are indicated and color-coded. (b, c) Bar charts representing the effect of the pan-NOS inhibitor L-NAME (magenta) (ChIn: 250 µM, ventral: 1000 µM), the NOS1 inhibitor 3-Bromo-7-nitroindazole (blue) (3Br-7NI) (20 µM) and the sGC inhibitor ODQ (orange) (20 µM) on inflammation of the peripheral sensory nervous system assessed by the ChIn assay (b) and on epithelial inflammation assessed by mechanically induced injury of the ventral fin (c). Bar charts represent means of the inflammatory index (%) ± SEM. Three individual experiments were performed with 15 replicate larvae for each condition, respectively. Statistics were evaluated with unpaired one-sided t-tests. ns-not significant: P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001.