FIGURE

Fig. S1

ID

ZDB-FIG-151229-9

Publication

Isaacman-Beck et al., 2015 - The lh3 Glycosyltransferase Directs Target-Selective Peripheral Nerve Regeneration

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Fig. S1

Conditional expression of Tg(hsp70:lh3myc) rescues lh3 motor axon guidance defects, related to Figures 2 and 3.

(A) Schematic showing conditional expression paradigm.

(B) Tg(hsp70:lh3myc) is no longer detectable by western blot at 4 dpf.

(C,D) Schematics showing patterns of dorsal and ventral motor axon outgrowth detected in (E-G) and (H-J) with Tg(isl1:gfp) and Tg(mnx1:gfp) respectively.

(E-G) Dorsal (72hpf) and (H-J) ventral (52hpf) motor axon development was rescued by expression of Tg(hsp70:lh3myc) (n > 20 larvae, 200 nerves per condition). Red arrowheads show misguided mutant axons.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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