ZFIN ID: ZDB-FIG-151214-64
Mikami et al., 2015 - Mindbomb 2 is dispensable for embryonic development and Notch signalling in zebrafish. Biology Open   4(11):1576-82 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 1-4 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 1-4 somites

Fig. 3

Mib2 deficiency does not significantly affect early neurogenesis and Notch signalling. Whole-mount in situ hybridization using elavl3 (A) and her4.1 (B) antisense probes at the 3 somite stage, showing no difference in neurogenesis phenotypes between wild-type and mib2cd3 embryos or between the mib1 mutants and mib1/mib2 double mutants. All panels show top views of embryos with the anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
elavl3 WT control 1-4 somites neuroblast ISH
mib1ta52b/ta52b standard conditions 1-4 somites neuroblast ISH
mib1ta52b/ta52b ; mib2chi3/chi3 standard conditions 1-4 somites neuroblast ISH
mib1tfi91/tfi91 standard conditions 1-4 somites neuroblast ISH
mib2chi3/chi3 standard conditions 1-4 somites neuroblast ISH
mib2chi3/chi3 ; mib1tfi91/tfi91 standard conditions 1-4 somites neuroblast ISH
her4.1 WT control 1-4 somites neuron ISH
mib1ta52b/ta52b standard conditions 1-4 somites neuron ISH
mib1ta52b/ta52b ; mib2chi3/chi3 standard conditions 1-4 somites neuron ISH
mib1tfi91/tfi91 standard conditions 1-4 somites neuron ISH
mib2chi3/chi3 standard conditions 1-4 somites neuron ISH
mib2chi3/chi3 ; mib1tfi91/tfi91 standard conditions 1-4 somites neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mib1ta52b/ta52b standard conditions 1-4 somites neuroblast elavl3 expression increased amount, abnormal
1-4 somites neuron her4.1 expression decreased amount, abnormal
mib1ta52b/ta52b ; mib2chi3/chi3 standard conditions 1-4 somites neuroblast elavl3 expression increased amount, abnormal
1-4 somites neuron her4.1 expression decreased amount, abnormal
mib1tfi91/tfi91 standard conditions 1-4 somites neuroblast elavl3 expression increased amount, abnormal
1-4 somites neuron her4.1 expression decreased amount, abnormal
mib2chi3/chi3 standard conditions 1-4 somites neurogenesis process quality, normal
1-4 somites Notch signaling pathway process quality, normal
mib2chi3/chi3 ; mib1tfi91/tfi91 standard conditions 1-4 somites neuroblast elavl3 expression increased amount, abnormal
1-4 somites neuron her4.1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open