FIGURE

Fig. S2

ID

ZDB-FIG-151208-9

Publication

Teh et al., 2015 - Modulating expression level of secreted Wnt3 influences cerebellum development in zebrafish transgenics

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Fig. S2

Cells expressing Wnt3EGFP in the midbrain hindbrain boundary and cerebellum are located in proliferation niches. (A) An in vivo image of a double transgenic larvae co-expressing the membrane localized KillerRed in the midbrain hindbrain boundary (mhb) and cerebellum of Tg(memKR15-16) and C terminal EGFP Wnt3 fusion proteins from Tg(wnt3:Wnt3EGFP)^F2. Double transgenic larvae confirmed Wnt3EGFP expression in the mhb and the cerebellum. wnt3:Wnt3EGFP+ cells in the cerebellum are predominantly localized to the upper rhombic lip (url). (B-E)Wnt3EGFP cells at midbrain hindbrain boundary (mhb) are glial cells (B) as it stained positively for GFAP (glial fibrilary acidic protein) and are negative for the neuronal marker HuC/D (C-E). (F-G) Midline restricted Wnt3EGFP cells at midbrain hindbrain boundary (demarcated by membrane KR expression in this domain) are proliferating cells since they stained positively for Brdu.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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