Fig. S5
Wnt9b overexpression reduces dorsal/neural tissues. (a)-(d) 1-cell aged embryos were injected with either 140 pg or 280 pg of synthetic Wnt9b mRNA and monitored for survival (e) and 48hpf eye phenotypes (f). Live embryos scored for (a) normal eyes, (b) small eyes, (c) cyclopia, and (d) no eye phenotypes that are represented in (f). (b) Blue columns are the 0 - 1dpf survival, red columns are the 1 - 2dpf survival. (f) Distribution of eye phenotypes of Wnt9b overexpressing embryos. Purple = no eyes, green = cyclopic, red = small eyes, blue = normal eyes. |