Fig. 4

Kif5Ba localizes Buc to cleavage furrows to mediate germ plasm assembly in the embryo. (A,B) GFP-Buc is recruited to distal furrows of WT (arrowheads in A) but not of Mkif5Ba mutants (B). [n=0/29 for Mkif5Ba^ae11/ae12 (N=2 females), n=0/18 for Mkif5Ba^ae11/ae11 (N=3 females), n=0/15 for kif5Ba^ae12/ae12 (N=1 female), n=32/32 for WT (N=4 females) have compact distal GFP-Buc; n=2/29 for Mkif5Ba^ae11/ae12, n=15/18 for Mkif5Ba^ae11/ae11, n=0/15 for Mkif5Ba^ae12/ae12 have some furrow recruitment of GFP-Buc, although distal furrow compaction is defective.] (C,D) Endogenous Buc localizes to distal cleavage furrows of WT (arrowheads in C) but not Mkif5Ba mutants (D). [n=2/15 for Mkif5Ba^ae12/ae12 (N=2 females), n=2/17 for Mkif5Ba^ae11/ae11 (N=1 female), n=24/32 for WT (N=4 females) of cells with decondensed chromosomes have Buc at e1 distal cleavage furrow.] (E-H) Compared with uninjected WT (E), gfp-buc injected WT (F) embryos have additional nanos3⁺ PGCs (arrowheads), whereas uninjected (G) and gfp-buc-injected (H) Mkif5Ba mutants lacked nanos3⁺ cells. See text for quantification.