FIGURE

Fig. 6

ID
ZDB-FIG-151021-8
Publication
Yoon et al., 2015 - First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio)
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Fig. 6

Zebrafish CD4-1 protein expression in transfected CHO cells and zebrafish tissues.

(A) FACS analysis of zfCD4-1 positive cells in CHO cells transfected with zfCD4-1. Cells reacted with only secondary antibody or the pre-immune rabbit serum and secondary antibody are shown in the black and grey histogram, respectively, whilst cells incubated with zfCD4-1 polyclonal antibody and secondary antibody are shown in the red histogram. Note the increase in fluorescence intensity of the latter, as shown by a shift to the right. (B) and (C), Western blot analysis of zfCD4-1 protein. (B) Un-transfected CHO cell lysate (5 μl) (lane 1), and zfCD4-1 transfected CHO cell lysates (5μl, lane 2; 10μl, lane 3). (C), zf blood cell lysate (lane 1), kidney lysate (lane 2) and muscle lysate (lane 3). The data are representative of 3 independent experiments.
Expression Data
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
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