FIGURE

Fig. 7

ID

ZDB-FIG-150929-42

Publication

Rajaram et al., 2014 - Technical brief: Constant intense light exposure to lesion and initiate regeneration in normally pigmented zebrafish

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Fig. 7

Light damage triggers tuba1a:GFP transgene expression in Müller glia (MG). A: tg:1016tuba1a:GFP zebrafish were exposed to the constant intense light paradigm, and retinas were collected at the indicated time points and assessed for GFP expression (red) by immunohistochemistry. GFP⁺ cells began to appear in the INL by 24 h (arrowheads). By 72 h, clusters of GFP⁺ cells were detected in the INL and ONL (double arrowheads). B: Immunohistochemistry for GFP and GS in 51-h light-exposed 1016tuba1a:GFP retinas. Arrows indicate GS⁺ and GFP⁺ MG. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; IPL, inner plexiform layer. Scale bars are 50 µm.

Expression Data

Gene:	GFP
Fish:	mi4Tg
Condition:	high light intensity
Anatomical Term:	Muller cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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