FIGURE

Fig. 4

ID

ZDB-FIG-150827-13

Publication

Sun et al., 2014 - Human Kidney Disease-causing INF2 Mutations Perturb Rho/Dia Signaling in the Glomerulus

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Fig. 4

INF2 knockout leads to altered podocyte structure and nephrin mistrafficking. a. Ultrastructure of glomerulus and podocyte in INF2 morphant zebrafish vs. control fish. b. Cultured podocytes were transfected with INF2-targeting siRNA or a control RNA duplex (control siRNA) for 48 h. Then the cells in both groups were transfected with a plasmid encoding human nephrin. 24 h later, nephrin expression was illustrated by immunofluorescence stain in cultured podocyte with INF2 knockdown (INF2 siRNA) vs. control cells (control siRNA). c. Glomerular section of INF2 morphants (INF2 MO) and control fish (control MO). The expression of nephrin is illustrated using immunofluorescence staining in the zebrafish glomerulus (INF2 morphants (INF2 MO) vs. control fish (control MO)).

Expression Data

Gene:	nphs1
Antibody:	AB1-nphs1
Fish:	WT WT + MO1-inf2
Knockdown Reagent:	MO1-inf2
Anatomical Term:	pronephric glomerulus
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-inf2
Knockdown Reagent:	MO1-inf2
Observed In:	glomerular basement membrane microvillus glomerular basement membrane podocyte foot glomerular basement membrane slit diaphragm
Stage:	Day 4

Phenotype Detail

Acknowledgments

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