FIGURE

Fig. 6

ID

ZDB-FIG-150811-33

Publication

Vanhollebeke et al., 2015 - Tip cell-specific requirement for an atypical Gpr124- and Reck-dependent Wnt/β-catenin pathway during brain angiogenesis

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Fig. 6

Tip cell defects in Wnt/β-catenin-deficient PHBCs.

(A) Scatterplot of a microarray comparison of wild-type and gpr124^s984/s984 embryos at 48 hpf. Each dot refers to the relative signal intensity of a given probe in mutant vs wild-type embryos. Circles identify independent probes for vegfaa, vegfab, and vegfc. (B) Endothelial cell number in the PHBCs of wild-type and gpr124^s984/s984 embryos at 30 hpf. kdrl:NLS-GFP⁺ nuclei were counted on one side of the embryos. (C) Dorsal views of Tg(pdgfrb:mCitrine);Tg(kdrl:ras-mCherry) wild-type and gpr124^s984/s984 embryos at 40 hpf. (D) Stills from Video 3 recording sprouting angiogenesis in the hindbrain of Tg(kdrl:GFP) wild-type and gpr124^s984/s984 embryos. Below are kymograph plots showing a time course of pixel intensity across the yellow virtual lines running 15 µm dorsal to the PHBC (from the anterior MCeV to the posterior PCeV [posterior cerebral vein]) and depicted in the upper left panel. (E) Lateral views of Tg(kdrl:lifeact-GFP);Tg(kdrl:NLS-mCherry) wild-type and gpr124^s984/s984 hindbrains at 30 hpf. Arrows point to actin-dense structures forming in the PHBC vessel wall. Scale bars, 50 µm. Values represent means ± SD.

Expression Data

Genes:	Citrine EGFP mCherry
Fish:	s843Tg is4Tg; s982Tg s896Tg; ubs1Tg s1010Tg; s896Tg adgra2^s984/s984; is4Tg; s982Tg adgra2^s984/s984; s843Tg adgra2^s984/s984; s896Tg; ubs1Tg adgra2^s984/s984; s1010Tg; s896Tg
Anatomical Terms:	basilar artery brain vasculature cranial vasculature endothelial cell filamentous actin mid cerebral vein mid cerebral vein endothelial cell pericyte posterior communicating artery posterior communicating artery anatomical region posterior communicating artery pericyte primordial hindbrain channel primordial hindbrain channel angiogenic sprout primordial hindbrain channel endothelial cell primordial hindbrain channel filamentous actin
Stage Range:	Prim-15 to High-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	adgra2^s984/s984; is4Tg; s982Tg adgra2^s984/s984; s843Tg adgra2^s984/s984; s896Tg; ubs1Tg
Observed In:	endothelial cell filamentous actin primordial hindbrain channel angiogenic sprout primordial hindbrain channel dorsal region primordial hindbrain channel endothelial cell primordial hindbrain channel endothelial tip cell fate specification primordial hindbrain channel sprouting angiogenesis
Stage Range:	Prim-15 to High-pec

Phenotype Detail

Acknowledgments

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