FIGURE

Fig. 5

ID

ZDB-FIG-150721-7

Publication

Huysseune et al., 2014 - Wnt signaling during tooth replacement in zebrafish (Danio rerio): pitfalls and perspectives

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Fig. 5

Figure 5. Enhanced Wnt signaling through LiCl treatment. Dentition of control zebrafish at start of treatment at 45 hpf (A), and treated with 300 mM LiCl for 1 h (B, fixed at 60 hpf and C, fixed at 112 hpf). In (B,C) only tooth 4V¹ is present. Dentition of control zebrafish at start of treatment at 72 hpf (D), and treated with 300 mM LiCl for 1 h (E, fixed at 92 hpf), or treated with 300 mM LiCl for 10 min (F, fixed at 112 hpf). (E) All three first-generation teeth (3V¹, 4V¹, and 5V¹) have formed and replacement tooth 4V² is in morphogenesis stage (note that the section is slightly oblique and different sets of teeth are visible on either body side); (F) several tooth buds appear to form from a single enamel organ (black arrowhead, inset). Color codes for arrowheads as in Figure 3. Scale bars = 50 µm for A-F and 20 µm for inset.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment: lithium chloride
Observed In:	primary dental epithelium tooth 3V tooth 4V tooth 5V tooth placode
Stage Range:	Pec-fin to Day 5

Phenotype Detail

Acknowledgments

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