FIGURE

Fig. 4

ID

ZDB-FIG-150626-2

Publication

Xu et al., 2015 - Characterization of Tetratricopeptide Repeat-Containing Proteins Critical for Cilia Formation and Function

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Examinations on cilia in the Kupffer’s vesicle and pronephric duct.

(A-C) Both cilia number and cilia length in the Kupffer’s vesicle (KV) at the 7-somite stage were reduced in the indicated ttc morphants. Cilia were labeled using antibody against acetylated tubulin. The quantification results were based on three independent experiments. Student’s t-test, ***P < 0.001. Error bars represent s.d. (D) Typical morphology of cilia in pronephric ducts at 24 hpf. (E) and (F) Cilia motility in the pronephric duct at 60 hpf. The kymographs showed trajectories of the cilia marked with red line in S1, S2, S3, S4, S5 and S6 Videos. Cilia beat frequencies (CBFs) were measured at 60 hpf (40 cilia from 4 morphants for each gene). Student’s t-test, ***P < 0.001. Error bars represent s.e.m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-odad4 WT + MO1-ttc4 WT + MO1-ttc9c WT + MO1-ttc36 WT + MO1-ttc39c
Knockdown Reagents:	MO1-odad4 MO1-ttc4 MO1-ttc9c MO1-ttc36 MO1-ttc39c
Observed In:	cilium movement Kupffer's vesicle cilium pronephric duct motile cilium
Stage Range:	5-9 somites to Pec-fin

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One