FIGURE

Fig. 5

ID

ZDB-FIG-150618-2

Publication

Palpant et al., 2015 - Cardiac Development in Zebrafish and Human Embryonic Stem Cells Is Inhibited by Exposure to Tobacco Cigarettes and E-Cigarettes

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Fig. 5

Analysis of hESC derived fetal cardiomyocyte transcription factor, calcium handling, and junction protein expression.

(a) Expression level of cardiac transcription factors GATA4 and NKX2.5 (a) and calcium handling proteins including the L-type calcium channel and SERCA2a, and the junctional protein CNX43 (b) by quantitative RT-PCR in cells treated with 6.8 �M e-cigarette or tobacco cigarette extracts vs. control. (c-d) Representative immunocytochemistry (c) and quantification (e) for NKX2.5 in fetal cardiomyocytes with various cigarette treatments compared to control. (e-f) Representative immunohistochemistry (e) and quantification (f) for the junction protein cadherin in fetal hESC cardiomyocytes with various cigarette treatments compared to control. Inset shown to the right. Arrows indicate perinuclear expression of cadherin. n ≥ 6 per group. Scale bar = 100 �m. * P < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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