ZFIN ID: ZDB-FIG-150505-4
Lin et al., 2015 - Angiopoietin-like proteins stimulate HSPC development through interaction with Notch receptor signaling. eLIFE   4 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Prim-25

Fig. 2 Angptls genetically interact with notch.

(A) Notch eGFP Tg reporter embryos injected with angptls-MO (2 ng) had decreased notch activity in ISV (black arrowheads) and DA (red arrowhead) at 28hpf. (B) Overexpression of constitutively active notch in angptls-MO injected (2 ng) Tg(hsp70:Gal4;UAS:NICD) can restore cmyb-positive HSPCs (arrows) at 36hpf after heatshock. (C) Mib had no cmyb-positive HSPCs (arrows) at 36hpf compared to WT or het siblings. Overexpression of zangptl2 in mib by crossing Tg(hsp70:zangptl2) into mib can restore this defect upon heatshock. Scale bars: 50 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP um14Tg control Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
um14Tg + MO1-angptl1a + MO2-angptl2b control Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
myb WT heat shock Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
WT + MO1-angptl1a + MO2-angptl2b heat shock Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
cz3328Tg heat shock Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
cz3328Tg ; mib1ta52b/ta52b heat shock Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
kca3Tg; kca4Tg heat shock Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
kca3Tg; kca4Tg + MO1-angptl1a + MO2-angptl2b heat shock Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
mib1ta52b/ta52b heat shock Prim-25 Not Detected ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-angptl1a + MO2-angptl2b heat shock Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell myb expression decreased distribution, abnormal
Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell absent, abnormal
cz3328Tg heat shock Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell myb expression increased distribution, abnormal
Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell increased amount, abnormal
cz3328Tg ; mib1ta52b/ta52b heat shock Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell myb expression amount, ameliorated
Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell amount, ameliorated
kca3Tg; kca4Tg heat shock Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell myb expression increased distribution, abnormal
Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell increased amount, abnormal
kca3Tg; kca4Tg + MO1-angptl1a + MO2-angptl2b heat shock Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell myb expression amount, ameliorated
Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell amount, ameliorated
mib1ta52b/ta52b heat shock Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell myb expression absent, abnormal
Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell absent, abnormal
um14Tg + MO1-angptl1a + MO2-angptl2b control Prim-5 dorsal aorta EGFP expression decreased amount, abnormal
Prim-5 intersegmental vessel EGFP expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife