Fig. 6

Dynamin inhibition disrupts the organization of the adherens junction. ((A)(I)) Localization of adherens junction components β-Catenin ((A)(C)) and E-Cadheren ((D)(F)), and apical membrane marker aPKC ((G)(I)) in untreated ((A), (D) and (G)), DMSO control treated ((B), (E) and (H)) and dynasore treated ((C), (F) and (I)) embryos. Confocal projections from the animal pole are shown in ((A)(I)). Arrowheads in ((C), (F) and (I)) indicate cells with enriched staining at the junction between cells. Arrows in (I) indicate folds in the apical membrane. Enlarged representative XZ planes showing the localization of each protein with respect to the apicalbasal axis of the EVL are shown in ((A′)(I′)). Membranes are marked with mGFP for reference and apical is to the top. White dots are placed approximately above the intercellular junctions. Arrowheads indicate regions of aberrant antibody staining.