Fig. 5

EVL-biased DN-Dnm2 expression disrupts cell morphology, cell packing and ZO-1 localization. ((A) and (B)) EVL cell morphology in embryos mosaically expressing GFP alone (cntl inj, (A)(A′′)) or GFP and DN-Dnm2l ((B)(B′′)). Phalloidin staining is shown in ((A) and (B)), GFP-marked clones in ((A′) and (B′)) and overlay in ((A′′) and (B′′)). Arrowheads in ((A′) and (B′)) show deep cells that have been labeled. Arrow in (B′′) indicates a DN-Dnm2l expressing cell which is abnormally small, lacks its characteristic polygonal shape and contacts a neighbouring cell at a vertex instead of along its edge. ((C) and (D)) Quantification of the effect of DN-Dnm2l on EVL cell apical surface area and cell packing. ((E) and (F)) ZO-1 localization in GFP ((E) and (E′)) and DN-Dnm2l ((F) and (F′)) expressing clones. Arrowheads in (E) indicate regions of reduced and discontinuous ZO-1 antibody staining.