FIGURE

Fig. 1

ID

ZDB-FIG-150422-57

Publication

Hochgreb-Hägele et al., 2015 - Znf385C mediates a novel p53-dependent transcriptional switch to control timing of facial bone formation

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Fig. 1

Expression of Citrine in Gt(znf385c-citrine)^ct39a depicts nuclear localization of endogenous Znf385C in zebrafish. (A) Schematic representation of znf385c splice variants. Exons in the full-length (v1) or unique to the splice variant (v2) are shown in dark and light blue, respectively. Orange bars: zinc finger domains. Gray bars: target region for anti-sense riboprobe. (B–H) Left side (B, D–G) and ventral views (C). (B and C) Onset of Znf385C expression at 3 dpf in the chondrocytes of the jaws, ears and fins. (D–G) Confocal imaging of live embryos shows nuclear localization (green) of Znf385C in the chondrocytes of the face (D) and ears (E). Low expression level of Znf385C in the branchial arches (F) and dorsal aspect of neural tube (G) at 3 dpf. Counterstaining by BODIPY-TR (red). (H–J) Confocal live imaging of zebrafish chondrocytes at 3 dpf. (H) Expression of endogenous Znf385C in Gt(znf385c-citrine)^ct39a. mRNA expression of Znf385C variants V1 (I) and V2 (J). White arrows point punctate pattern condensations for endogenous Znf385C and V1, while V2 is localized in the nuclear periphery. (K–M) Expression of znf385c splice variants by whole mount in situ hybridization at 56 hpf. Lateral views. Black arrows point to areas of znf385c expression. (K) v1 expression in the olfactory region and ventral aspect of the forebrain and hindbrain. (L) v2 is more diffuse, with stronger distribution in the brain ventricle, ears and pharyngeal region. (M) Endogenous znf358c is detected as a fusion with citrine in Gt(znf385c)^ct39a line. mc, Meckel’s cartilage; e: eye; ov, otic vesicle; f, fin; ba, branchial arches; nt, neural tube; o, olfactory placode; v, ventricle; h, heart.

Expression Data

Gene:	Citrine
Fish:	znf385c^ct39aGt
Anatomical Terms:	central nervous system neuron hindbrain inner ear chondrocyte mandibular arch skeleton chondrocyte pectoral fin chondrocyte pharyngeal arch 3-7
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 400(1), Hochgreb-Hägele, T., Koo, D.E., Bronner, M.E., Znf385C mediates a novel p53-dependent transcriptional switch to control timing of facial bone formation, 23-32, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.