FIGURE

Fig. 1

ID

ZDB-FIG-150422-26

Publication

Zhang et al., 2015 - Spindle Assembly Checkpoint Acquisition at the Mid-Blastula Transition

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Fig. 1

Pre-MBT embryos lack a functional SAC.

Embryos were injected with Alexa 647-Histone H1 and PCNA-GFP proteins, treated with or without nocodazole before the MBT at 2 HPF, then imaged live. Images show cell cycle progression based on Histone H1 and PCNA from an embryo at 2.75 HPF. Insets are displayed with higher contrast settings to show the changing morphology of a single nucleus at different cell cycle stages: I, interphase; M, prometaphase/metaphase; A, anaphase. In the montage, time between metaphase and the next interphase is 8 min for untreated control embryos and 9 min for nocodazole-treated embryos. Graph shows average length of mitosis for each condition (ne14 embryos for each, from three independent experiments). Error bars indicate S.D.; P>0.05; two-tailed Student’s t test; scale bars 20 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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