FIGURE
Fig. 3
- ID
- ZDB-FIG-150422-17
- Publication
- Hisano et al., 2015 - Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish
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Fig. 3
Precise genome modification using CRISPR/Cas9 with our donor vector is heritable. (a) An F1 embryo was obtained by mating wild-type fish with the F0 founder fish injected with eGFP-gRNA, krtt1c19e-gRNA, Cas9 mRNA and the donor vector, and it exhibited eGFP expression in the epidermis at 2 dpf. (b) The numbers of F0 fish screened and of founders bearing eGFP-positive progeny are indicated. (c) Genomic DNA was prepared from the embryo expressing eGFP, and sequence analysis confirmed the precise integration of eGFP into the krtt1c19e locus. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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