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Fig. 3
Precise genome modification using CRISPR/Cas9 with our donor vector is heritable.
(a) An F1 embryo was obtained by mating wild-type fish with the F0 founder fish injected with eGFP-gRNA, krtt1c19e-gRNA, Cas9 mRNA and the donor vector, and it exhibited eGFP expression in the epidermis at 2 dpf. (b) The numbers of F0 fish screened and of founders bearing eGFP-positive progeny are indicated. (c) Genomic DNA was prepared from the embryo expressing eGFP, and sequence analysis confirmed the precise integration of eGFP into the krtt1c19e locus.
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