Fig. 1

Unique regeneration defects in the clo mutant. (A) Impaired fin fold regeneration in the clo mutant and normal regeneration in the sibling wild-type. The arrowheads mark the characteristic blister-like structures often observed in the amputated mutant tissue. The dashed line indicates the site of amputation. (B) TUNEL analysis of amputated fin fold of clo mutant and sibling wild-type at 12 hpa. The graph in the right shows the quantification of TUNEL-positive cells in the clo mutant (n=25) and wild-type (n=28) in the regeneration-responsive region (brackets). (C) BrdU analysis of cell proliferation. The BrdU labeling was performed 0?24 hpa. The graph shows the quantification of BrdU-positive cells in clo mutant (n=7) and wild-type (n=7) in the bracketed regions. Data are presented as mean±s.e.m. Statistical significance was tested using Welchós t test. **P<0.001. The dotted lines indicate the outline of the fin fold. Scale bars for clo and wild-type represent 50 µm, respectively. dpa, days post amputation; hpa, hours post amputation.