PUBLICATION
A diffusible signal derived from hematopoietic cells supports the survival and proliferation of regenerative cells during zebrafish fin fold regeneration
- Authors
- Hasegawa, T., Nakajima, T., Ishida, T., Kudo, A., Kawakami, A.
- ID
- ZDB-PUB-141224-20
- Date
- 2015
- Source
- Developmental Biology 399(1): 80-90 (Journal)
- Registered Authors
- Kawakami, Atsushi, Kudo, Akira
- Keywords
- Apoptosis, Fin fold, Myeloid, Regeneration, Zebrafish, cloche, tal1
- MeSH Terms
-
- Cell Survival/genetics
- Cell Survival/physiology
- Larva/cytology
- Larva/metabolism
- Larva/physiology
- Microscopy, Confocal
- Regeneration/genetics
- Regeneration/physiology*
- Proto-Oncogene Proteins/genetics
- Proto-Oncogene Proteins/metabolism
- Animal Fins/injuries
- Animal Fins/physiology*
- Mutation
- In Situ Hybridization
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Basic Helix-Loop-Helix Transcription Factors/metabolism
- Wound Healing/genetics
- Wound Healing/physiology
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish/physiology
- Signal Transduction/genetics
- Signal Transduction/physiology*
- Animals
- Animals, Genetically Modified
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Apoptosis/genetics
- Apoptosis/physiology
- Cell Proliferation*
- Hematopoietic Stem Cells/metabolism*
- PubMed
- 25533245 Full text @ Dev. Biol.
Citation
Hasegawa, T., Nakajima, T., Ishida, T., Kudo, A., Kawakami, A. (2015) A diffusible signal derived from hematopoietic cells supports the survival and proliferation of regenerative cells during zebrafish fin fold regeneration. Developmental Biology. 399(1):80-90.
Abstract
Multicellular organisms maintain body integrity by constantly regenerating tissues throughout their lives; however, the overall mechanism for regulating regeneration remains an open question. Studies of limb and fin regeneration in teleost fish and urodeles have shown the involvement of a number of locally activated signals at the wounded site during regeneration. Here, we demonstrate that a diffusible signal from a distance also play an essential role for regeneration. Among a number of zebrafish mutants, we found that the zebrafish cloche (clo) and tal1 mutants, which lack most hematopoietic tissues, displayed a unique regeneration defect accompanying apoptosis in primed regenerative tissue. Our analyses of the mutants showed that the cells in the primed regenerative tissue are susceptible to apoptosis, but their survival is normally supported by the presence of hematopoietic tissues, mainly the myeloid cells. We further showed that a diffusible factor in the wild-type body fluid mediates this signal. Thus, our study revealed a novel mechanism that the hematopoietic tissues regulate tissue regeneration through a diffusible signal.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping