Fig. 1

Angiogenesis inhibition by WA-25 in vivo and ex vivo (A) Chemical structures of WA-25 (dihydroaustrasulfone alcohol) and WE-2 (austrasulfone); (B) Effect of WA-25 on intersegmental vessels (ISVs) development in transgenic Tg(fli-1:EGFP)^y1 zebrafish embryos. (Left panel) Representative photographs of ISV in control and WA-25 (50 µM)-treated zebrafish at 48 h poat-fertilization (hpf). Magnification 40×; scale bar, 50 µm. (Right panel) Quantification analysis of enhanced green fluorescent protein (EGFP) intensities in the ISVs in WA-25 (50 µM)-treated embryos. Data are represented as mean ± SEM (n = 12); (C) Effect of WA-25 (50 µM) on SIV development in transgenic Tg(fli-1:EGFP)^y1 zebrafish embryos. Photographs of control and WA-25-treated zebrafish were taken at 72 hpf. Magnification, 100×; scale bar, 100 µm. Asterisks indicate arcades in the vesicle-like structure (D) Effect of WA-25 on microvessel sprouting in aortic rings. Rat aortic rings were placed in Matrigel and treated with WA-25 (20 µM) for 7 days. Scale bar, 2 mm. Data are represented as mean ± SEM (n = 12). ** p < 0.01.