FIGURE

Fig. 4

ID

ZDB-FIG-150406-53

Publication

Nelson et al., 2014 - Global identification of Smad2 and Eomesodermin targets in zebrafish identifies a conserved transcriptional network in mesendoderm and a novel role for Eomesodermin in repression of ectodermal gene expression

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Fig. 4

Eomesa and Foxh1 combinatorially regulate early mesendoderm marker expression and are required for endoderm formation. In situ hybridisation of ntla(A-E), gsc(F-L) and sox32(M-S) at 30% epiboly in wild-type embryos, foxh1 morphants, MZeomesa mutants, MZeomesa;foxh1 morphants, MZoep mutants, and in wild type embryos and MZeomesa mutants injected with foxh1 mRNA. Rescued expression of sox32 expression in the ventral margin of MZeomesa mutants injected with foxh1 mRNA is indicated by an asterisk. In situ hybridisation of ttna(T-W) at 24 hpf in wild-type embryos, foxh1 morphants, MZeomesa mutants and MZeomesa;foxh1 morphants showing expression in the heart (arrow heads) and somites. The open arrow heads indicate cardia bifida. Animal views; dorsal to the right. Numbers on each panel indicate the number of embryos showing the phenotype depicted over the total number of embryos analysed. For sox32 expression in MZoep mutants faint staining in the YSL was detected in 11/15 embryos as previously reported [54]. hpf, hours post fertilization.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biol.