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Fig. 4 Eomesa and Foxh1 combinatorially regulate early mesendoderm marker expression and are required for endoderm formation. In situ hybridisation of ntla(A-E), gsc(F-L) and sox32(M-S) at 30% epiboly in wild-type embryos, foxh1 morphants, MZeomesa mutants, MZeomesa;foxh1 morphants, MZoep mutants, and in wild type embryos and MZeomesa mutants injected with foxh1 mRNA. Rescued expression of sox32 expression in the ventral margin of MZeomesa mutants injected with foxh1 mRNA is indicated by an asterisk. In situ hybridisation of ttna(T-W) at 24 hpf in wild-type embryos, foxh1 morphants, MZeomesa mutants and MZeomesa;foxh1 morphants showing expression in the heart (arrow heads) and somites. The open arrow heads indicate cardia bifida. Animal views; dorsal to the right. Numbers on each panel indicate the number of embryos showing the phenotype depicted over the total number of embryos analysed. For sox32 expression in MZoep mutants faint staining in the YSL was detected in 11/15 embryos as previously reported [54]. hpf, hours post fertilization.