FIGURE

Fig. 5

ID

ZDB-FIG-150402-5

Publication

Kok et al., 2015 - Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish

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Fig. 5

Normal Hindbrain Development in megamind Mutant Embryos

(A) Schematic of the megamind locus in wild-type and megamind^um209 mutants. The red and green boxes indicate relative position of the TALEN target sequences, and the MO target sequence is shown.

(B) RT-PCR for the megamind and cyrano lincRNAs as well as eef1al1 in wild-type and megamind^um209 mutant embryos. RT refers to RNA template without () or with (+) reverse transcription.

(C–F) Transmitted light images of the head region in embryos at 48 hrpf. The hindbrain ventricle is indicated with an arrow in each image. Lateral views, anterior to the left, and dorsal is up. (C) Wild-type, uninjected; (D) megamind^um209 mutant; (E) wild-type injected with 20 ng megamind conserved site MO; and (F) megamind^um209 mutant injected with megamind conserved site MO.

Expression Data

Genes:	birc6-as2 eef1a1l1 oip5-as1
Fish:	WT birc6-as2^um209/um209
Anatomical Term:	whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	birc6-as2^um209/um209 birc6-as2^um209/um209 + MO3-birc6-as2 WT + MO3-birc6-as2
Knockdown Reagent:	MO3-birc6-as2
Observed In:	fourth ventricle whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 32(1), Kok, F.O., Shin, M., Ni, C., Gupta, A., Grosse, A.S., van Impel, A., Kirchmaier, B.C., Peterson-Maduro, J., Kourkoulis, G., Male, I., DeSantis, D.F., Sheppard-Tindell, S., Ebarasi, L., Betsholtz, C., Schulte-Merker, S., Wolfe, S.A., Lawson, N.D., Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish, 97-108, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell