Fig. 8

Notch inhibitor canceled the HDAC effect on cell proliferation in the optic tectum of adult zebrafish. A: Expression of her4 in transverse sections of the adult zebrafish optic tectum with or without 400 nM TSA treatment. Scale bar = 500 µm (a-d), 100 µm (a′-d′). CCe, corpus cerebelli; PGZ, periventricular gray zone; TeO, tectum opticum. B: Quantitative RT-PCR for her4 in the optic tectum of adult zebrafish with or without 400 nM TSA treatment. C: Quantitative RT-PCR for her4 in the optic tectum of adult zebrafish with or without 5 µM LY411575 treatment. D: Quantitative RT-PCR for her4 in the optic tectum of adult zebrafish with or without 500 µM VPA and 5 µM LY411575 treatment. E: Number of PCNA-positive cells in total slices (P = 0.89; control: n = 4; TSA + Notch inhibitor: n = 3). F: Number of PCNA-positive cells in 10 caudal slices (P = 0.81; control: n = 4; TSA + Notch inhibitor: n = 3). *P < 0.05.