FIGURE

Fig. 4

ID

ZDB-FIG-150326-39

Publication

Dozawa et al., 2014 - Valproic acid, a histone deacetylase inhibitor, regulates cell proliferation in the adult zebrafish optic tectum

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Fig. 4

VPA decreases cell proliferation in the optic tectum of adult zebrafish. A: Timeline of drug treatment and method of quantification of cell number. B: Immunostaining with PCNA and BrdU in the dorsomedial area of the PGZ of the adult zebrafish optic tectum. Scale bar = 50 µm. C: The number of PCNA-positive cells (control: n = 3, VPA: n = 4). D: The number of BrdU-positive cells (control: n = 3; VPA: n = 4). E: The number of PCNA-positive cells in 10 caudal slices (control: n = 3; VPA: n = 4). F: The number of BrdU-positive cells in 10 caudal slices; 500 µM VPA treatment reduces the number of proliferating cells (control: n = 3; VPA: n = 4). G: Timeline of drug treatment. H: Immunostaining with HuC and BrdU in the PGZ of the adult zebrafish optic tectum. Scale bar = 100 µm. I: The number of BrdU-positive cells in 10 caudal slices (P = 0.26; control: n = 4; VPA: n = 3). J: The percentage of BrdU and HuC double-positive cells in BrdU-positive cells in 10 caudal slices. VPA administration does not relate to the promotion of neuronal differentiation (P = 0.32; control: n = 4; VPA: n = 3). CCe, corpus cerebelli; PGZ, periventricular gray zone; TeO, tectum opticum. **P < 0.01, *P < 0.05.

Expression Data

Antibody:	Ab2-pcna
Fish:	WT
Condition:	chemical treatment: pharmaceutical
Anatomical Term:	periventricular grey zone dorso-medial region
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment: pharmaceutical
Observed In:	optic tectum cell population proliferation
Stage:	Adult

Phenotype Detail

Acknowledgments

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