FIGURE

Fig. 2

ID

ZDB-FIG-150309-35

Publication

Angers et al., 2014 - Itch Is Required for Lateral Line Development in Zebrafish

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Fig. 2

Expression of itch genes in zebrafish embryos.

(A) RT-PCR experiment showing early expression of both itcha and itchb genes. RNA was extracted from embryos at approximately 6, 24 and 48 hpf. cDNA was obtained from each stage using 1 µg total RNA. GAPDH was used as an internal control for cDNA amplification. (B–D) Weak, general staining of embryos at 26 hpf revealed with DIG-labelled probes complementary to itcha (B) or itchb (C) or sense sequence (D) as a control. (E–G) The primordium of 26 hpf embryos revealed with DIG-labelled probes complementary to itcha (E) or itchb (F) or sense sequence (G) as a control. Position of the primordium is underlined with a dotted line. Expression of itch genes is general, and itchb is expressed throughout the primordium. Scale bar: (B–D) 250 µm, (E–G) 25 µm.

Expression Data

Genes:	itcha itchb
Fish:	WT
Anatomical Terms:	posterior lateral line primordium whole organism
Stage Range:	Shield to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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