ZFIN ID: ZDB-PUB-141105-1
Itch Is Required for Lateral Line Development in Zebrafish
Angers, A., Drapeau, P.
Date: 2014
Source: PLoS One   9: e111799 (Journal)
Registered Authors: Drapeau, Pierre
Keywords: none
MeSH Terms:
  • Animals
  • Cell Movement
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Humans
  • Lateral Line System*
  • Phylogeny
  • Ubiquitin-Protein Ligases/genetics*
  • Ubiquitin-Protein Ligases/metabolism
  • Wnt Signaling Pathway
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 25369329 Full text @ PLoS One
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ABSTRACT
The zebrafish posterior lateral line is formed during early development by the deposition of neuromasts from a migrating primordium. The molecular mechanisms regulating the regional organization and migration of the primordium involve interactions between Fgf and Wnt/[Formula: see text]-catenin signaling and the establishment of specific cxcr4b and cxcr7b cytokine receptor expression domains. Itch has been identified as a regulator in several different signaling pathways, including Wnt and Cxcr4 signaling. We identified two homologous itch genes in zebrafish, itcha and itchb, with generalized expression patterns. By reducing itchb expression in particular upon morpholino knockdown, we demonstrated the importance of Itch in regulating lateral line development by perturbing the patterns of cxcr4b and cxcr7b expression. Itch knockdown results in a failure to down-regulate Wnt signaling and overexpression of cxcr4b in the primordium, slowing migration of the posterior lateral line primordium and resulting in abnormal development of the lateral line.
ADDITIONAL INFORMATION