ZFIN ID: ZDB-FIG-150127-14
Hüsken et al., 2014 - Tcf7l2 Is Required for Left-Right Asymmetric Differentiation of Habenular Neurons. Current biology : CB   24(19):2217-27 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Pec-fin to Day 4

Fig. 5

The Symmetric dHb Phenotype of tcf7l2 Mutants Is Epistatic to Parapineal Ablation

Dorsal views focused on the epithalamus of 2.5 dpf (A and A′) and 4 dpf (B–E′) embryos with anterior to the top.

(A and A′) The parapineal (pseudocolored red) is on the left in both wild-type and tcf7l2 mutant embryos.

(B–E′) In Et(gata2a:EGFP)pku588; Tg(foxD3:GFP); Tg(flh:eGFP) triple-transgenic normal and parapineal-ablated embryos, dHbl neurons (green) and the pineal complex are labeled. The pineal is pseudocolored in blue and the parapineal and its projections in red (B–C′). Embryos as in (B)–(C′) were labeled for kctd12.1 subsequent to live imaging (D–E′).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP pku588Et; u711Tg; zf104Tg standard conditions Day 4 dorsal habenular nucleus IHC
Day 4 epiphysis IHC
u711Tg; zf104Tg standard conditions Pec-fin epiphysis IHC
GFP pku588Et; u711Tg; zf104Tg standard conditions Day 4 parapineal organ IHC
u711Tg; zf104Tg standard conditions Pec-fin parapineal organ IHC
kctd12.2 WT standard conditions Day 4 habenula ISH
tcf7l2u754/u754 standard conditions Day 4 habenula ISH
tcf7l2u763/u763 standard conditions Day 4 habenula ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.