FIGURE

Fig. 6

ID

ZDB-FIG-150122-8

Publication

Zada et al., 2014 - Altered Behavioral Performance and Live Imaging of Circuit-Specific Neural Deficiencies in a Zebrafish Model for Psychomotor Retardation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Loss of MCT8 reduces synaptic density in axonal arbors of the motor neuron. A-C. Confocal imaging of a 2 dpf live tg(mct8:EGFP) embryo co-injected with huc:GAL4 and uas:tRFP constructs revealed co-localization of mct8 (green) and the huc pan-neural marker (red) in a motor neuron. D. Schematic illustration of an axonal arbor in a motor neuron. Each color represents a single branch that was subjected to ImageJ software analysis. E. Lateral view of a 3 dpf tg(huc:GAL4Xuas:memYFP) embryo. memYFP expression driven by the huc promoter is observed in the spinal cord (SC) and in descending motor neurons. The dashed frame marks a single motor neuron that was selected for further comparative studies. High magnification of the framed area is shown in the trunk of 6 dpf tg(huc:GAL4Xuas:memYFP)/mct8+/- and tg(huc:GAL4Xuas:memYFP)/mct8-/- representative larvae (F and G, respectively). H. Lateral view of a 30 hpf tg(mct8:GAL4Xuas:SYP-EGFP) embryo. SYP-EGFP expression driven by the mct8 promoter is observed in the spinal cord (SC) and in descending motor neurons. In order to compare the number of synapses in mct8+/- and mct8-/- larvae, single motor-neuron arbors were selected (dashed frame). High magnification of the dashed frame is shown in 6 dpf tg(mct8:GAL4)/(uas:SYP-EGFP)/mct8+/- and tg(mct8:GAL4)/(uas:SYP-EGFP)/mct8-/- representative larvae (I and J, respectively). The total arbor length (K) and the number of branches (L) were measured in 3 and 6 dpf mct8+/- larvae and in 3 and 6 dpf mct8-/- larvae. M. Synapse density in the axons of the motor-neurons was measured along the last 50 µm of a single branch in 3 and 6 dpf mct8+/- larvae and in 3 and 6 dpf mct8-/- larvae. N. The total number of synapses was measured in the motor-neuron arbor of 6 dpf mct8+/- and mct8-/- larvae. Scale bar = 30 µm. Values represented as means±SEM (standard error of the mean). Statistical significance determined by t-test: Two-sample assuming unequal variances followed by one-sample Kolmogorov-Smirnov test, to assume normal distribution (*p<0.05).

Expression Data

Genes:	EGFP YFP
Fish:	biu2Tg mde4Tg; mde6Tg biu3Tg; biu5Tg slc16a2^biu4/biu4; mde4Tg; mde6Tg slc16a2^biu4/biu4; biu3Tg; biu5Tg
Anatomical Terms:	motor neuron motor neuron neuron projection motor neuron synapse spinal cord
Stage Range:	Prim-15 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc16a2^biu4/biu4; biu3Tg; biu5Tg
Observed In:	motor neuron neuron projection motor neuron synapse
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.