ZFIN ID: ZDB-FIG-141007-72
Wagh et al., 2014 - Fam40b is required for lineage commitment of murine embryonic stem cells. Cell Death & Disease   5:e1320 Full text @ Cell Death Dis.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 7

The in situ hybridization of Fam40b in the zebrafish heart as well as the expression of vmhc and of cardiac cmlc2 in control and Fam40b knockdown animals. (a) Digoxigenin-labeled RNA probes were prepared using RNA labeling kit and stained using BM purple. (b) The position of the atrium (arrowhead) and the ventricle (arrow) that was not beating after knockdown of Fam40b is shown. (c) Expression of vmhc in Fam40b knockdown animals was significantly impaired as compared with control animals (d) (arrows show the ventricles). Expression of cmlc2 in Fam40b knockdown animals was almost absent in ventricles and impaired in the atria (e) as compared with control (f) (upper arrows indicate ventricles and lower arrows indicate atria; one representative experiment out of five independent). Whole mount embryos were imaged on a Leica stereomicroscope fitted with a Zeiss Axiocam color camera

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myh7l WT control Long-pec cardiac ventricle ISH
WT + MO1-strip1 standard conditions Long-pec cardiac ventricle ISH
myl7 WT control Long-pec atrium ISH
Long-pec cardiac ventricle ISH
WT + MO1-strip1 standard conditions Long-pec atrium ISH
Long-pec Not Detected cardiac ventricle ISH
strip1 WT standard conditions Long-pec cardiac ventricle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO1-strip1 standard conditions Long-pec atrium increased size, abnormal
Long-pec blood accumulation cardiac ventricle, abnormal
Long-pec heart decreased functionality, abnormal
Long-pec heart vasculature non-functional, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Death & Disease for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Death Dis.