|ZFIN ID: ZDB-FIG-140923-14|
Mbp and acetylated tubulin staining after knock-down of the zebrafish orthologue, exosc8 in the head
Un-injected control larvae and exosc8 MO injected larvae were analysed for myelination at 96 hpf. Larvae of the different phenotype categories and control larvae were stained with antibodies against the myelin basic protein (Mbp) and against acetylated tubulin to study the presence of axon tracts and their degree of myelination.
Left column: overlay, with Mbp staining in red and acetylated tubulin staining in green; middle column: Mbp staining; right column: acetylated tubulin. Top row: head of control larva. Middle row: MO injected larva with normal external morphology; axons near the eye are not Mbp-positive (white arrowhead).
Bottom row: MO injected larva with a severe phenotype.
|mbpa||Ab1-mbp||slc24a5unspecified||standard conditions||Day 4||head||IHC|
|Ab1-mbp||slc24a5unspecified + MO1-exosc8||standard conditions||Day 4||head||IHC|
|slc24a5unspecified + MO1-exosc8||standard conditions||Day 4||brain development disrupted, abnormal|
|Day 4||hindbrain morphology, abnormal|
|Day 4||myelination disrupted, abnormal|
|Acknowledgments:||ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.|