FIGURE

Fig. S3

ID

ZDB-FIG-140923-14

Publication

Boczonadi et al., 2014 - EXOSC8 mutations alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia

Other Figures

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Fig. S3

Mbp and acetylated tubulin staining after knock-down of the zebrafish orthologue, exosc8 in the head

Un-injected control larvae and exosc8 MO injected larvae were analysed for myelination at 96 hpf. Larvae of the different phenotype categories and control larvae were stained with antibodies against the myelin basic protein (Mbp) and against acetylated tubulin to study the presence of axon tracts and their degree of myelination.

Left column: overlay, with Mbp staining in red and acetylated tubulin staining in green; middle column: Mbp staining; right column: acetylated tubulin. Top row: head of control larva. Middle row: MO injected larva with normal external morphology; axons near the eye are not Mbp-positive (white arrowhead).

Bottom row: MO injected larva with a severe phenotype.

Expression Data

Gene:	mbpa
Antibody:	Ab1-mbp
Fish:	slc24a5^unspecified slc24a5^unspecified + MO1-exosc8
Knockdown Reagent:	MO1-exosc8
Anatomical Term:	head
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc24a5^unspecified + MO1-exosc8
Knockdown Reagent:	MO1-exosc8
Observed In:	brain development hindbrain myelination
Stage:	Day 4

Phenotype Detail

Acknowledgments

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