FIGURE

Fig. 5

ID

ZDB-FIG-140915-31

Publication

Law et al., 2014 - The Serine-Threonine Protein Kinase PAK4 Is Dispensable in Zebrafish: Identification of a Morpholino-Generated Pseudophenotype

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Fig. 5

Testing the ability of mutant RNAs to rescue the MO knockdown phenotype.

(A) Whole mount in situ hybridization of wild type and mutant embryos at 30 hpf for mpo. (B) MZpak4 knockdown-rescue data. Percentages of embryos showing undetectable, reduced or normal staining as compared to control for mpo expression at 30 hpf in (A). UI, uninjected wild type EK. MO, 6 ng pak4 MO cocktail. MO+wt, 6 ng pak4 MO cocktail plus 800 pg wild type PAK4-eGFP RNA. MO+Δ2, 6 ng pak4 MO cocktail plus 800 pg PAK4Δ2-eGFP RNA. MO+Δ4, 6 ng pak4 MO cocktail plus 800 pg PAK4Δ4-eGFP RNA. Only the wild type pak4 RNA resulted in significant rescue of mpo expression.

Expression Data

Gene:	mpx
Fish:	WT WT + MO3-pak4 + MO4-pak4 + MO5-pak4
Knockdown Reagents:	MO3-pak4 MO4-pak4 MO5-pak4
Anatomical Terms:	granulocyte hematopoietic system
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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