FIGURE

Fig. 5

ID

ZDB-FIG-140903-40

Publication

Schiavone et al., 2014 - Zebrafish reporter lines reveal in vivo signaling pathway activities involved in pancreatic cancer

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Fig. 5

TGFβ, Notch, Shh and Bmp signaling activities during pancreatic cancer onset. TGFβ, Notch, Shh and Bmp signaling activities were evaluated by observing mCherry expression using confocal microscope analysis. (A–D) Pictures of full Z-stacks from 30 and 60 dpf samples. Controls obtained by outcrossing the Tg(ptf1a:eGFP) zebrafish line with TGFβ (A) and Notch (C) mCherry reporter lines. TGFβ (B) and Notch (D) signaling activity in tumor lines. (E–H) Quantitative analysis of eGFP-KRAS^G12D-positive cells expressing TGFβ (E), Notch (F), Shh (G) and Bmp (H) mCherry reporters by VOLOCITY 6.0 software. For each time point, 10 eGFP-positive samples were analyzed. Results for TGFβ (E) and Notch (F) signaling were statistically significant. Error bars indicate s.e.m. for all analyzed samples; **P<0.01, ***P<0.005 according to ANOVA test.

Expression Data

Genes:	EGFP mCherry
Fish:	ia7Tg/ia7Tg; jh16Tg ia15Tg; jh16Tg
Anatomical Term:	pancreas
Stage Range:	Days 30-44 to Days 45-89

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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