FIGURE

Fig. 3

ID

ZDB-FIG-140903-1

Publication

Da Costa et al., 2014 - A new zebrafish model of SOD1 ALS produced by TILLING replicates key features of the disease and represents a tool for in vivo therapeutic screening

Other Figures

Fig. 3

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Fig. 3

Neuromuscular junction analysis. (A) 11-dpf WT larva stained with α-bungarotoxin conjugated with Alexa Fluor 488. (B) 11-dpf WT larva stained with SV2 (from Developmental Studies Hybridoma Bank, University of Iowa, IA, USA) and Alexa Fluor 594. (C) 11-dpf homozygous T70I larva stained with α-bungarotoxin conjugated with Alexa Fluor 488. (D) 11-dpf homozygous T70I larva stained with SV2 and Alexa Fluor 594. Images have been background corrected. (E) Colocalisation analysis was completed using the WCIF suite of plug-ins for ImageJ. Colocalisation r-values from the interseptal regions of WT, T70I heterozygous (Het) and T70I homozygous (Hom) 11-dpf larvae from a heterozygous incross. Statistical significance of difference between means was determined by one-way ANOVA and Dunn’s multiple comparison test (n=30). *P<0.05; ***P<0.001.

Expression Data

Antibody:	Ab-SV2
Fish:	WT sod1^zf503/zf503
Anatomical Term:	trunk neuromuscular junction
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sod1^zf503/zf503
Observed In:	trunk neuromuscular junction
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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