ZFIN Image: Da Costa et al., 2014, Fig. 3

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Figure Caption

Fig. 3

Neuromuscular junction analysis. (A) 11-dpf WT larva stained with α-bungarotoxin conjugated with Alexa Fluor 488. (B) 11-dpf WT larva stained with SV2 (from Developmental Studies Hybridoma Bank, University of Iowa, IA, USA) and Alexa Fluor 594. (C) 11-dpf homozygous T70I larva stained with α-bungarotoxin conjugated with Alexa Fluor 488. (D) 11-dpf homozygous T70I larva stained with SV2 and Alexa Fluor 594. Images have been background corrected. (E) Colocalisation analysis was completed using the WCIF suite of plug-ins for ImageJ. Colocalisation r-values from the interseptal regions of WT, T70I heterozygous (Het) and T70I homozygous (Hom) 11-dpf larvae from a heterozygous incross. Statistical significance of difference between means was determined by one-way ANOVA and Dunn’s multiple comparison test (n=30). *P<0.05; ***P<0.001.

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Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.