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ZIRC
ZFIN ID: ZDB-FIG-140902-3
Al-Hamed et al., 2014 - Functional modelling of a novel mutation in BBS5. Cilia   3:3 Full text @ Cilia
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 4

bbs5 morphants display cardiac anomalies including situs inversus. Wildtype (WT) and morphant cmlc2:GFP embryos were imaged at 48 hpf. (A) WT heart showing normal looping (D-looping) with the atrium (a) on the left hand side of the embryo and the ventricle (v) on the right. (B) Morphant heart with preserved D-looping. (C) Morphant heart with looping in the opposite direction (L-looping) resulting in the atrium on the right of the embryo and the ventricle on the left. (D) Morphant heart, which shows a failure of looping, resulting in a heart with neither the atrium nor ventricle leaving the midline. (E) 98% of WT embryos displayed D-looping whilst just 61% of morphant embryos exhibited D-looping, 12% exhibited L-looping, and 27% of morphant hearts were unlooped (P < 0.001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg standard conditions Long-pec atrium IFL
Long-pec cardiac ventricle IFL
Long-pec heart IFL
twu34Tg + MO2-bbs5 standard conditions Long-pec atrium IFL
Long-pec cardiac ventricle IFL
Long-pec heart IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
twu34Tg + MO2-bbs5 standard conditions Long-pec heart looping process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cilia for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cilia