|ZFIN ID: ZDB-FIG-140811-44|
Def haploinsufficiency caused an altered migration behaviour of leukocytes in def+/- fish that was rescued by treatment with Dex or ectopic expression of Def in hepatocytes.
(A–H) Representative images of co-immunostaining of EGFP (green, label macrophage), dsRed (yellow, label neutrophil) and Bhmt (red) in the wound epidermis in wild-type (A,B), def+/- (C,D), def+/- treated with Dex (E,F) and def+/-Tg(fabp10a:def) progeny (G,H) 12 and 36 h after PH, respectively. All are in the background of Tg(coro1a:eGFP;lyz:dsRED). Nuclei were stained with DAPI (blue). In each case, more than 10 sections from at least three wild-type or def+/- mutant fish were examined. bc, blood clot; lv, liver tissue. Scale bars: 150 μm (A–H).
|Ab1-bhmt||IHC||hkz04tTg; nz50Tg||physical alteration: anatomical structure||Adult||hepatocyte|
|IHC||utp25hi429Tg/+; hkz04tTg; nz50Tg||physical alteration: anatomical structure||Adult||hepatocyte|
|IHC||utp25hi429Tg/+; hkz04tTg; nz50Tg; zju1Tg||physical alteration: anatomical structure||Adult||hepatocyte|
|utp25hi429Tg/+; hkz04tTg; nz50Tg||physical alteration: anatomical structure||Adult||inflammatory response to wounding process quality, abnormal|
|Acknowledgments:||ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One|