ZFIN ID: ZDB-FIG-140811-44
Zhu et al., 2014 - Haploinsufficiency of Def Activates p53-Dependent TGFbeta Signalling and Causes Scar Formation after Partial Hepatectomy. PLoS One   9:e96576 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 8

Def haploinsufficiency caused an altered migration behaviour of leukocytes in def+/- fish that was rescued by treatment with Dex or ectopic expression of Def in hepatocytes.

(A–H) Representative images of co-immunostaining of EGFP (green, label macrophage), dsRed (yellow, label neutrophil) and Bhmt (red) in the wound epidermis in wild-type (A,B), def+/- (C,D), def+/- treated with Dex (E,F) and def+/-Tg(fabp10a:def) progeny (G,H) 12 and 36 h after PH, respectively. All are in the background of Tg(coro1a:eGFP;lyz:dsRED). Nuclei were stained with DAPI (blue). In each case, more than 10 sections from at least three wild-type or def+/- mutant fish were examined. bc, blood clot; lv, liver tissue. Scale bars: 150 μm (A–H).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 hkz04tTg; nz50Tg physical alteration: anatomical structure Adult neutrophil IHC
utp25hi429Tg/+; hkz04tTg; nz50Tg physical alteration: anatomical structure Adult neutrophil IHC
utp25hi429Tg/+; hkz04tTg; nz50Tg; zju1Tg physical alteration: anatomical structure Adult neutrophil IHC
EGFP hkz04tTg; nz50Tg physical alteration: anatomical structure Adult macrophage IHC
Adult neutrophil IHC
utp25hi429Tg/+; hkz04tTg; nz50Tg physical alteration: anatomical structure Adult macrophage IHC
Adult neutrophil IHC
utp25hi429Tg/+; hkz04tTg; nz50Tg; zju1Tg physical alteration: anatomical structure Adult macrophage IHC
Adult neutrophil IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-bhmt IHC hkz04tTg; nz50Tg physical alteration: anatomical structure Adult hepatocyte
IHC utp25hi429Tg/+; hkz04tTg; nz50Tg physical alteration: anatomical structure Adult hepatocyte
IHC utp25hi429Tg/+; hkz04tTg; nz50Tg; zju1Tg physical alteration: anatomical structure Adult hepatocyte
Phenotype Details
Fish Conditions Stage Phenotype
utp25hi429Tg/+; hkz04tTg; nz50Tg physical alteration: anatomical structure Adult inflammatory response to wounding process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One