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General Information
ZIRC
ZFIN ID: ZDB-FIG-140808-16
Qiao et al., 2014 - Snail modulates the assembly of fibronectin via alpha5 integrin for myocardial migration in zebrafish embryos. Scientific Reports   4:4470 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 1 (A, B): Phenotypes of snai1b knockdown at 48hpf, lateral view. Black arrows: heart valve. (C–F): Changes in heart shape in myl7::GFP embryos injected with control (Ctl) and snai1b MO at 48hpf and 72hpf. A, atrium; V, ventricle. (G): Quantification of phenotypes produced by snai1b morphants at 48hpf, with or without snai1b mRNA at different concentrations. (H, I): Heart endocardium in flk::GFP embryos injected with Ctl and snai1b MO at 72hpf. White arrows: heart. Scale bars: 100µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP s843Tg control Long-pec to Protruding-mouth endocardium IFL
s843Tg + MO1-snai1b standard conditions Long-pec to Protruding-mouth endocardium IFL
twu34Tg control Long-pec atrium IFL
Long-pec cardiac ventricle IFL
Long-pec heart IFL
Protruding-mouth atrium IFL
Protruding-mouth cardiac ventricle IFL
Protruding-mouth heart IFL
twu34Tg + MO1-snai1b standard conditions Long-pec atrium IFL
Long-pec cardiac ventricle IFL
Long-pec heart IFL
Protruding-mouth atrium IFL
Protruding-mouth cardiac ventricle IFL
Protruding-mouth heart IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-snai1b standard conditions Long-pec heart looping process quality, abnormal
Long-pec pericardium edematous, abnormal
s843Tg + MO1-snai1b standard conditions Long-pec - Protruding-mouth endocardium development process quality, normal
twu34Tg + MO1-snai1b standard conditions Long-pec atrium position, abnormal
Long-pec cardiac ventricle decreased size, abnormal
Long-pec heart looping process quality, abnormal
Protruding-mouth atrium position, abnormal
Protruding-mouth cardiac ventricle decreased size, abnormal
Protruding-mouth heart looping process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.