PUBLICATION
Snail modulates the assembly of fibronectin via alpha5 integrin for myocardial migration in zebrafish embryos
- Authors
- Qiao, L., Gao, H., Zhang, T., Jing, L., Xiao, C., Xiao, Y., Luo, N., Zhu, H., Meng, W., Xu, H., Mo, X.
- ID
- ZDB-PUB-140513-279
- Date
- 2014
- Source
- Scientific Reports 4: 4470 (Journal)
- Registered Authors
- Mo, Xianming, Zhang, Ting
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Movement/genetics
- Embryo, Nonmammalian
- Embryonic Development*
- Fibronectins/genetics
- Fibronectins/metabolism*
- Heart/growth & development
- Integrin alpha5/genetics
- Integrin alpha5/metabolism*
- Morphogenesis/genetics
- Myocardium/cytology
- Transcription Factors/antagonists & inhibitors
- Transcription Factors/genetics*
- Zebrafish
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics*
- PubMed
- 24667151 Full text @ Sci. Rep.
Citation
Qiao, L., Gao, H., Zhang, T., Jing, L., Xiao, C., Xiao, Y., Luo, N., Zhu, H., Meng, W., Xu, H., Mo, X. (2014) Snail modulates the assembly of fibronectin via alpha5 integrin for myocardial migration in zebrafish embryos. Scientific Reports. 4:4470.
Abstract
The Snail family member snail encodes a zinc finger-containing transcriptional factor that is involved in heart formation. Yet, little is known about how Snail regulates heart development. Here, we identified that one of the duplicated snail genes, snai1b, was expressed in the heart region of zebrafish embryos. Depletion of Snai1b function dramatically reduced expression of α5 integrin, disrupted Fibronectin layer in the heart region, especially at the midline, and prevented migration of cardiac precursors, resulting in defects in cardiac morphology and function in zebrafish embryos. Injection of α5β1 protein rescued the Fibronectin layer and then the myocardial precursor migration in snai1b knockdown embryos. The results provide the molecular mechanism how Snail controls the morphogenesis of heart during embryonic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping