FIGURE

Fig. 4

ID

ZDB-FIG-140807-71

Publication

He et al., 2014 - Role of histone deacetylase activity in the developing lateral line neuromast of zebrafish larvae

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Fig. 4

The effect of HDAC inhibitors on the levels of histone acetylation. (a) Western blot analysis of protein extracts from 5dpf control larvae and larvae treated with HDAC inhibitors for 2 days starting at 3dpf. HDAC inhibitors induced the accumulation of acetylated (Ace) H3 and H4. Histones H3 and H4 were included as controls. (b) Confocal images of neuromasts from a 5dpf control larva and from 5dpf larvae treated with TSA, VPA or MS-275. Larvae were treated with 0.1μM TSA, 100μM VPA or 10μM MS-275 for 2 days starting at 3dpf and were stained (red) by antibodies against Ace histone H3 and Ace histone H4. The levels of Ace histone H3 and Ace histone H4 are increased in the presence of HDAC inhibitors compared with the controls (CON). HCs were labeled with antibodies against Myosin VI (green) and nuclei were stained with DAPI (blue). Scale bar=10μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

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