FIGURE

Fig. 2

ID

ZDB-FIG-140807-13

Publication

Burger et al., 2014 - A zebrafish model of chordoma initiated by notochord-driven expression of HRASV12

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Fig. 2

Immunohistochemical features of the zebrafish notochord tumors. 7-dpf larvae were examined by immunohistochemical techniques. The left two columns show control and twhh:Gal4;UAS:HRASV12, and the right two columns show control and 4465:Gal4;UAS:HRASV12. (A–D) pERK staining in control embryos (A,C) showed minimal nuclear positivity in the notochord cells. EGFP-HRASV12 embryos showed focal, strong nuclear and weak cytoplasmic positivity in tumor cells (B,D). (E–H) pS6 staining in control embryos was negative in the notochord (E,G), but the tumor cells showed strong nuclear and weak cytoplasmic positivity. (I–L) Cytokeratin staining showed cytoplasmic positivity in normal notochord cells (I,K) and in the tumor (J,L). (M–P) Brachyury staining demonstrated nuclear and, to a lesser extent, cytoplasmic positivity in normal notochord cells (M,O) and in the tumor (N,P). (Q–T) Corresponding histology of normal notochord (Q,S) and the notochord tumor (R,T).

Expression Data

Antibodies:	Ab1-t Ab2-krt Ab4-rps6 Ab10-mapk
Fish:	zf279Et fb201Tg; io006Tg io006Tg; zf279Et
Anatomical Terms:	notochord notochord cytoplasm notochord nucleus
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fb201Tg; io006Tg io006Tg; zf279Et
Observed In:	notochord notochord chordoma
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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