FIGURE

Fig. 5

ID

ZDB-FIG-140804-23

Publication

Elks et al., 2014 - Mycobacteria Counteract a TLR-Mediated Nitrosative Defense Mechanism in a Zebrafish Infection Model

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Fig. 5

Increased tyrosine nitration following Mm infection is dependent on MyD88.

(A) Fluorescent micrographs of confocal images showing the overlap between mpx:GFP and anti-nitrotyrosine staining in myd88^+/+ or myd88^-/- embryos. (B) Example fluorescence confocal z-stacks of the caudal vein region of myd88^+/+ or myd88^-/- embryos stained with anti-nitrotyrosine antibody, imaged at 1 dpi in the presence or absence of Mm infection. (C) Corrected fluorescence intensity levels of anti-nitrotyrosine antibody confocal z-stacks of equal size 1 day after injection with Mm in myd88^+/+ or myd88^-/- embryos. Data shown are mean ± SEM, n = 30 cells from 5 embryos representative of 2 independent experiments.

Expression Data

Gene:	GFP
Antibody:	Ab1-nitrotyrosine
Fish:	i114Tg myd88^{hu3568/hu3568}; i114Tg
Condition:	bacterial treatment
Anatomical Term:	neutrophil
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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