Fig. 5
Competence to respond to Eda signaling is distributed throughout the oropharyngeal cavity. (A and B) Confocal imaging of GFP fluorescence and C-F) in situ hybridization analysis of gfp RNA expression in NF-κB reporter zebrafish. Oral expression is limited to neuromasts and pharyngeal expression to lower tooth germs (arrow) in fish lacking the ef1α:eda transgene (A, C, and E). Presence of this transgene (B, D, and F) induces reporter expression throughout the oropharyngeal cavity, including in lower (arrow) and upper (arrowhead) pharyngeal tooth germs. Abbreviations: dpf, days postfertilization; e, eye; mc, Meckel’s cartilage; nm, neuromast. (Scale bars, 50 μm.) |
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Stage Range: | Day 4 to Day 5 |