Fig. S4

Tooth development is not induced in the mouth of ef1α:eda transgenic zebrafish. Comparison of gene expression in ventral views of the lower jaw of wild-type zebrafish (A, D, G, J, M, P), ef1α:eda transgenbic zebrafish (B, E, H, K, N, Q), and wild-type A. mexicanus (C, F, I, L, O, R). Arrowheads indicate location of tooth germs in A. mexicanus. (A–C) pitx2 is expressed in oral epithelium of A. mexicanus, with higher levels present in tooth germs (1). No expression corresponding to tooth germs is seen in wild type or ef1α:eda transgenic zebrafish, although oral expression is present. (D–F) Sonic hedgehog (shh) is expressed in oral epithelium of A. mexicanus, with higher levels present in tooth germs (1). No expression corresponding to tooth germs is seen in wild type or ef1α:eda transgenic zebrafish, although oral expression is present. (G–I) Bone morphogenetic protein 2b (bmp2b) is expressed in tooth germs of A. mexicanus (2). No expression corresponding to tooth germs is seen in wild-type or ef1α:eda transgenic zebrafish, although oral mesenchymal expression (2) is present. (J–L) The transcription factor dlx2b is expressed in tooth germs of A. mexicanus (1). No oral expression is seen in wild-type or ef1α:eda transgenic zebrafish. (M–O) Fibroblast growth factor 4 (fgf4) is expressed in tooth germs of A. mexicanus. No oral expression is seen in wild type or ef1α:eda transgenic zebrafish. (P–R) bmp2a is expressed in tooth germ mesenchyme in A. mexicanus (2). No oral expression is seen in wild-type or ef1α:eda transgenic zebrafish. Abbreviations: ch, ceratohyal; dpf, days postfertilization; mc, Meckel’s cartilage. (Scale bars, 50 &mu&m.)